The integrity and stability of specimens under different storage conditions for glucose-6-phosphate dehydrogenase deficiency screening using WST-8

• The first report comparing the stability of three different G6PD sample types (normal controls, heterozygous females and deficient individuals) under storage conditions. Specimens with normal activity, both EDTA blood DBS, can be stored at −20 °C for up to 1 year controlled Blood samples from as DBS conditions year. activity in significantly decreased over storage. Accurate measurement glucose-6-phosphate dehydrogenase (G6PD) is critical malaria treatment misclassification deficiency could cause serious harm patients. should assessed on day collection. Otherwise, specimens suitable prevent loss activity. Here, we integrity testing females, individuals using water-soluble tetrazolium salts (WST-8) assay. were ethylenediaminetetraacetic acid (EDTA) whole dried spots (DBS) various temperatures (37 °C, room temperature, 4 -20 °C) humidity (with without desiccant). stable when conditions, 85% 91% presence desiccant, respectively. showed greater enzyme after desiccant. rapidly lost all tested. However, reduction did not interfere classification. Samples will allow accurate enable safe effective use antimalarial drugs such primaquine tafenoquine.